Anti-Hu CD71 Biotin
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Product Specifications
Certification
RUO
Immunogen
NALM-6 human pre-B cell line
Target Antigen
CD71
Clone
MEM-75
Applications
FC (QC tested), IP, ICC
Concentration
1 mg/mL
Format
Biotin
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
Other References
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
Applications Notes
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Species
Human
CAS Number
9007-83-4
Isotype
Mouse IgG1 kappa
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