Anti-Hu CD79a APC

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta) . They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR) . CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Product Specifications

Certification

RUO

Reactivity

Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Non-human primates, Human

Immunogen

Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a

Target Antigen

CD79a

Clone

HM47

Applications

FC-IC (QC tested)

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128 (2) :306-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=bhargava+p+ross+js+azumi+n, *Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147 (11) :2474-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/1747162?ordinal, *Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95 (8) :2725-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/10753858 , *Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42 (4) :437-45., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=fernandez+nj+west+kh , *Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126 (7) :862-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/12123231 , *Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1 (1) :65-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hassan+a+perry+a+ning+y , *Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78 (24) :13891-900., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=netto+g+garcia+jv+cravens+pd

Other References

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a) - and Igbeta (CD79b) -mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177 (11) :7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina, *Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172 (4) :2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina, *Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194 (11) :1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.