Anti-Notch1 APC

Notch 1 is a 270-300 kDa transmembrane protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands such as DLL1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates into the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in bone marrow progenitor cells, brain, lung and thymus.

Product Specifications

Certification

RUO

Negative Species

Rat

Reactivity

Mouse, Human

Immunogen

GST fusion protein containing cdc10-NCR region of mouse Notch1

Target Antigen

Notch1

Clone

MN1A

Applications

FC-IC (QC tested)

Concentration

0.1 mg/mL

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120 (7) :2537-48., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=khwaja%20ss%20liu%20h Espinosa L Santos S Inglés-Esteve J Muñ oz-Canoves P Bigas A: p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear recept, *Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177 (4) :647-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/17502421

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.