Anti-Hu CD58 APC

CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

Product Specifications

Certification

RUO

Reactivity

Pig, Human

Immunogen

NALM-6 human pre-B cell line

Target Antigen

CD58

Clone

MEM-63

Applications

FC (QC tested)

Format

APC

Buffer

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Other References

*Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12 (26) :4237-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/16830383?ordin, *Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16 (6) :667-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15093607?ordin, *Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88 (11) :1245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/14607753?ordin, *Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84 (4) :2223-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/12668431?ordin

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.