Anti-Hu CD243 Purified Low Endotoxin

*Kelley DJ, Pavelic ZP, Gapany M, Stambrook P, Pavelic L, Gapany S, Gluckman JL: Detection of P-glycoprotein in squamous cell carcinomas of the head and neck. Arch Otolaryngol Head Neck Surg. 1993 Apr;119 (4) :411-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/8096141 , *Collnot EM, Baldes C, Schaefer UF, Edgar KJ, Wempe MF, Lehr CM: Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access. Mol Pharm. 2010 Jun 7;7 (3) :642-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20205474 , *Goda K, Fenyvesi F, Bacsó Z, Nagy H, Márián T, Megyeri A, Krasznai Z, Juhász I, Vecsernyés M, Szabó G Jr: Complete inhibition of P-glycoprotein by simultaneous treatment with a distinct class of modulators and the UIC2 monoclonal antibody. J Pharmacol Exp Ther. 2007 Jan;320 (1) :81-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17050779 , *Mechetner EB, Roninson IB: Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89 (13) :5824-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/1352877 , *Chaudhary PM, Mechetner EB, Roninson IB: Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80 (11) :2735-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=chaudhary+pm+mechetner+eb

The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.

Functional application: inhibition. Immunohistochemistry (paraffin sections) : Heat mediated antigen retrieval in citrate buffer pH 6. Flow cytometry: Recommended dilution: 1-4 µg/ml