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Anti-Ms NK1.1 Purified Low Endotoxin

NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

NK1-positive murine splenic and bone marrow cells

Target Antigen

NK1.1

Clone

PK136

Applications

FC (QC tested), IP, IHC-F, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Carlyle JR, Mesci A, Ljutic B, Belanger S, Tai LH, Rousselle E, Troke AD, Proteau MF, Makrigiannis AP: Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol. 2006 Jun 15;176 (12) :7511-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/16751398 , *GeurtsvanKessel CH, Bergen IM, Muskens F, Boon L, Hoogsteden HC, Osterhaus AD, Rimmelzwaan GF, Lambrecht BN: Both conventional and interferon killer dendritic cells have antigen-presenting capacity during influenza virus infection. PLoS One. 2009 Sep 28;4 (9) :e7187. , URL: http://www.ncbi.nlm.nih.gov/pubmed/19784375 , *Símová J, Bubeník J, Bieblová J, Jandlová T: The role of NK1.1+ cells in the protection against MHC class I+ HPV16-associated tumours. Folia Biol (Praha) . 2004;50 (6) :200-2., URL: http://www.ncbi.nlm.nih.gov/pubmed/15709715 , *Carroll JL, Nielsen LL, Pruett SB, Mathis JM: The role of natural killer cells in adenovirus-mediated p53 gene therapy. Mol Cancer Ther. 2001 Nov;1 (1) :49-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/12467238 , *Joncker NT, Shifrin N, Delebecque F, Raulet DH: Mature natural killer cells reset their responsiveness when exposed to an altered MHC environment. J Exp Med. 2010 Sep 27;207 (10) :2065-72., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=mature+natural+killer+cells+reset , *Wang M, Ellison CA, Gartner JG, HayGlass KT: Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses. J Immunol. 1998 Feb 1;160 (3) :1098-105., URL: http://www.ncbi.nlm.nih.gov/pubmed/9570522 , *Takahashi S, Kawamura T, Kanda Y, Taniguchi T, Nishizawa T, Iiai T, Hatakeyama K, Abo T: Activation of CD1d-independent NK1.1+ T cells in the large intestine by Lactobacilli. Immunol Lett. 2006 Jan 15;102 (1) :74-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16107279 , *Fang M, Lanier LL, Sigal LJ: A role for NKG2D in NK cell-mediated resistance to poxvirus disease. PLoS Pathog. 2008 Feb 8;4 (2) :e30., URL: http://www.ncbi.nlm.nih.gov/pubmed/18266471 , *Kulesza J, Hoser G, Wasilewska D, Kawiak J: NK cell depletion and recovery in SCID mice treated with anti-NK1.1 antibody. Folia Histochem Cytobiol. 2006;44 (2) :93-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kulesza+j+hoser+g , *Laffont S, Seillet C, Ortaldo J, Coudert JD, Guéry JC: Natural killer cells recruited into lymph nodes inhibit alloreactive T-cell activation through perforin-mediated killing of donor allogeneic dendritic cells. Blood. 2008 Aug 1;112 (3) :661-71, URL: http://www.ncbi.nlm.nih.gov/pubmed/18505782

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody PK136 recognizes an extracellular epitope of NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.

Applications Notes

Functional application: In vivo depletion of NK cells and NK-T cells. Flow cytometry: Recommended dilution: 1-4 µg/ml

Isotype

Mouse IgG2a kappa

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