Anti-Hu CD2 Purified Low Endotoxin

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Cytotoxic T lymphocytes

Target Antigen

CD2

Clone

TS1/8

Applications

FC (QC tested), FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA: Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A. 1982 Dec;79 (23) :7489-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/6984191 Vollger, *Zhang B, Dai M, Li QJ, Zhuang Y: Tracking proliferative history in lymphocyte development with cre-mediated sister chromatid recombination. PLoS Genet. 2013 Oct;9 (10) :e1003887., URL: http://www.ncbi.nlm.nih.gov/pubmed/24204301

Other References

*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/, *Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1 (10) :649-58., URL: https://pubmed.ncbi.nlm.nih.gov/17168569/, *Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272 (25) :15782-8., URL: https://pubmed.ncbi.nlm.nih.gov/9188475/, *Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody TS1/8 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.