Anti-Hu CD85j Purified Low Endotoxin

CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Hairy cell leukaemia cells

Target Antigen

CD85j

Clone

GHI/75

Applications

FC (QC tested), IP, WB, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Pulford K, Micklem K, Thomas J, Jones M, Mason DY: A 72-kD B cell-associated surface glycoprotein expressed at high levels in hairy cell leukaemia and plasma cell neoplasms. Clin Exp Immunol. 1991 Sep;85 (3) :429-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=clin+exp+immunol+85 (3) +pulford , *Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb;13 (2) :193-201., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=riteau+b+menier+c+dausset+j+martinozzi+s , *Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13 (9) :822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c , *Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun;65 (6) :841-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=banham+ah+colonna+m, *McIntire RH, Morales PJ, Petroff MG, Colonna M, Hunt JS: Recombinant HLA-G5 and -G6 drive U937 myelomonocytic cell production of TGF-beta1. J Leukoc Biol. 2004 Dec;76 (6) :1220-8., URL: https://pubmed.ncbi.nlm.nih.gov/15459235/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual) .