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Anti-Ms CD28 Purified Low Endotoxin

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

Mouse T cell lymphoma EL-4 cells

Target Antigen

CD28

Clone

37.51

Applications

FC (QC tested), IP, IHC-F, ICC, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Zhang et al.: The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway. J Immunol. 2008 Apr 15;180 (8) :5506-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/18390734?ordin, *Rosenberger CM, Clark AE, Treuting PM, Johnson CD, Aderem A: ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells. Proc Natl Acad Sci U S A. 2008 Feb 19;105 (7) :2544-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18268321?ordin, *Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS: The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007 Aug 1;179 (3) :1768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/17641043?ordin, *Pua HH, Dzhagalov I, Chuck M, Mizushima N, He YW: A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J Exp Med. 2007 Jan 22;204 (1) :25-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/17190837?ordin, *Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS: Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006 Aug;26 (16) :6005-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/16880512?ordin, *Zhang N, He YW: The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005 Jun 1;174 (11) :6967-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15905539?ordin, *Zhang N, He YW: An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med. 2005 Aug 1;202 (3) :395-404., URL: https://www.ncbi.nlm.nih.gov/pubmed/16043517?ordin, *Albert MH, Yu XZ, Martin PJ, Anasetti C: Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin. Blood. 2005 Feb 1;105 (3) :1355-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/15459004?ordin, *Gross JA, Callas E, Allison JP: Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992 Jul 15;149 (2) :380-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1320641?ordina, *Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP: CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature. 1992 Apr 16;356 (6370) :607-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1313950?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.

Applications Notes

Functional application: In vitro and in vivo T cell costimulation. Flow cytometry: Recommended dilution: 6 μg / ml. Immunocytochemistry: Recommended dilution: 1-4 μg / ml.Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 mL lysate.

Isotype

Golden Syrian Hamster IgG

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