Anti-Hu CD147 Purified Low Endotoxin

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer) .

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*Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 2000 Aug 1;19 (15) :3896-904., URL: https://www.ncbi.nlm.nih.gov/pubmed/10921872?ordin, *Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70) . J Biol Chem. 2005 Jul 22;280 (29) :27213-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/15917240?ordin, *Xu D, Hemler ME: Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug;4 (8) :1061-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/15901826?ordin, *Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T-cells by Impairing Vav1 and Rac1 Downstream Signaling. J Biol Chem. 2008 Feb 29;283 (9) :5554-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/18160397?ordin, *Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 2007 Dec;83 (3) :283-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/17945211?ordin, *Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin-mediated cell adhesion by a mechanism involving CD98-dependent activation of protein kinase C-delta and p44/42 mitogen-activated protein kinases. Exp Cell Res. 2008 Feb 1;314 (3) :616-28., URL: https://www.ncbi.nlm.nih.gov/pubmed/18054915?ordin, *Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE. Extracellular matrix metalloproteinase inducer (CD147) is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB-dependent inflammation in monocytes. Circ Res. 2008 Feb 15;102 (3) :302-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18048771?ordin

The antibody MEM-M6/6 recognizes extracellular Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.

Functional application: The antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation. Flow cytometry: recommended dilution: 1-4 μg/ml. Western blotting: Positive control: 293 human fibroblastoid cell line, non-reducing conditions.