Anti-Hu CD13 Purified Low Endotoxin

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

Human AML cells

Target Antigen

CD13

Clone

WM15

Applications

FC (QC tested), IP, IHC-F, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N) : predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21 (13) :1695-701. , URL: https://www.ncbi.nlm.nih.gov/pubmed/7902291?dopt=A, *McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121 (7) :e34-42. doi: 10.1182/blood-2012-05-429555., URL: https://www.ncbi.nlm.nih.gov/pubmed/23243270, *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987) ., URL: , *Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61 (1) :11-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/4052321?dopt=A, *Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17 (3) :392-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/3865147?dopt=A, *Weijler L, Kowarsch F, Wödlinger M, Reiter M, Maurer-Granofszky M, Schumich A, Dworzak MN: UMAP based anomaly detection for minimal residual disease quantification within acute myeloid leukemia. Cancers (Basel) 2022 Feb 11;14 (4) :898., URL: https://pubmed.ncbi.nlm.nih.gov/35205645/

Other References

*Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12 (13) :3971-8., URL: https://pubmed.ncbi.nlm.nih.gov/16818694/, *Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110 (1) :142-50., URL: https://pubmed.ncbi.nlm.nih.gov/17363739/, *Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140., URL: https://pubmed.ncbi.nlm.nih.gov/17655775/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.

Applications Notes

Functional application: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. Flow cytometry: Recommended dilution: 1-4 μg/ml.