Anti-Hu TCR Vbeta5.3-related Purified Low Endotoxin
Selected Variant
12-379-C100
Size: 0.1 mg
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Human thymoma cell line HPB-ALL.
Target Antigen
TCR Vbeta5.3-related
Clone
MEM-262
Applications
FC (QC tested), IP, WB, FUNC
Concentration
1 mg/mL
Format
Purified Low Endotoxin
Buffer
Phosphate buffered saline (PBS), pH 7.4
References & Citations
*Pavlistova D, Drbal K, Hilgert I, Horejsi V: A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett. 2003 Aug 5;88 (2) :105-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12880678?dopt=
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V (beta5.3) ] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V (beta5.3) -specific antibodies.
Applications Notes
Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting. Functional application: This antibody activates T cells (V beta 5-related subset) . Flow cytometry: Recommended dilution: 1-4 µg/ml
Isotype
Mouse IgG2a
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