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Anti-Hu TCR Vbeta5.3-related Purified Low Endotoxin

Selected Variant

12-379-C100

Size: 0.1 mg

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human thymoma cell line HPB-ALL.

Target Antigen

TCR Vbeta5.3-related

Clone

MEM-262

Applications

FC (QC tested), IP, WB, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Pavlistova D, Drbal K, Hilgert I, Horejsi V: A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett. 2003 Aug 5;88 (2) :105-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12880678?dopt=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V (beta5.3) ] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V (beta5.3) -specific antibodies.

Applications Notes

Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting. Functional application: This antibody activates T cells (V beta 5-related subset) . Flow cytometry: Recommended dilution: 1-4 µg/ml

Isotype

Mouse IgG2a

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