Anti-Transferrin Purified Low Endotoxin

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µ mol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

Product Specifications

Certification

RUO

Negative Species

Horse,Dog,Sheep,Cow

Reactivity

Human, Pig, Rabbit

Immunogen

Purified porcine transferrin.

Target Antigen

Transferrin

Clone

HTF-14

Applications

WB, IHC-P, ICC, ELISA, RIA, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Bartek J, Viklicky V, Franek F, Angelisova P, Draber P, Jarosikova T, Nemec M, Verlova H: Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity. Immunol Lett. 1982 May;4 (5) :231-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/7095835?dopt=A, *Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33 (1) :38-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/8824733?ordina, *Bartek J, Viklicky V, Stratil A: Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. Br J Haematol. 1985 Mar;59 (3) :435-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/2578807?dopt=A, *Viklický V, Bártek J: Utilization of monoclonal antibodies for mapping of antigenic determinants on the transferrin molecule. EOS n. 4, vol. 4, 1984, 263-267., URL:

Other References

*Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205 (4) :297-318., URL: https://www.ncbi.nlm.nih.gov/pubmed/15750326?ordin, *Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13 (35) :4725-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/17729394?ordin, *Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1 (3) :E79, URL: https://www.ncbi.nlm.nih.gov/pubmed/14691550?ordin, *Ghosh RN, Maxfield FR: Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells. J Cell Biol. 1995 Feb;128 (4) :549-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/7860630?ordina, *Ghosh RN, Gelman DL, Maxfield FR: Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy. J Cell Sci. 1994 Aug;107 (Pt 8) :2177-89., URL: https://www.ncbi.nlm.nih.gov/pubmed/7983176?ordina, *Hansen SH, Sandvig K, van Deurs B: Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification. J Cell Biol. 1993 Apr;121 (1) :61-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/8458873?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins) . It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Applications Notes

Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/ml.