Anti-Hu CD11a Purified Low Endotoxin

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Leukocytes from a patient suffering from a LGL-type leukaemia.

Target Antigen

CD11a

Clone

MEM-25

Applications

FC (QC tested), IP, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan;75 (1) :99-105., URL: https://www.ncbi.nlm.nih.gov/pubmed/14525961?itool, *Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18. J Immunol. 1999 Sep 1;163 (5) :2891-901., URL: https://www.ncbi.nlm.nih.gov/pubmed/10453036?itool, *Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., URL: https://www.ncbi.nlm.nih.gov/pubmed/26650353 , *Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83 (12) :6171-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/19339346?itool=, *Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93 (10) :3505-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/10233903?itool=, *Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4., URL: http://www.ncbi.nlm.nih.gov/pubmed/12659660?itool=, *Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95) . J Immunol. 2000 Jun 1;164 (11) :5928-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/10820275?itool=, *Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May;83 (9) :4195-204., URL: http://www.ncbi.nlm.nih.gov/pubmed/19211748?itool=, *Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar;9 (2) :403-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/11874886?itool, *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha) . 1990;36 (1) :41-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/1971601?dopt=A

Other References

*Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280 (31) :28290-8., URL: https://pubmed.ncbi.nlm.nih.gov/15955822/, *Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12 (22) :2771-95., URL: https://pubmed.ncbi.nlm.nih.gov/16918410/, *Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57., URL: https://pubmed.ncbi.nlm.nih.gov/17068975/, *Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80 (24-25) :2320-4., URL: https://pubmed.ncbi.nlm.nih.gov/17289088/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-25 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Applications Notes

Functional application: The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1. Immunoprecipitation: Excellent antibody for immunoaffinity purification of LFA-1 complex. Flow cytometry: Recommended dilution: 2 μg/ml.