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Anti-Hu CD137 Purified Low Endotoxin

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Product Specifications

Certification

RUO

Immunogen

Recombinant human CD137 ectodomain

Target Antigen

CD137

Clone

4B4-1

Applications

FC (QC tested), IP, ELISA, FUNC

Concentration

1 mg/mL

Format

Purified Low Endotoxin

Buffer

Phosphate buffered saline (PBS), pH 7.4

References & Citations

*Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int J Oncol. 2012 Oct;41 (4) :1486-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/22797667 , *Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol Cell Biol. 2012 Apr;90 (4) :449-56., URL: http://www.ncbi.nlm.nih.gov/pubmed/21747409 , *Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS One. 2014 Jan 21;9 (1) :e86337., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yi+l+zhao+y+wang+x , *Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models. J Mol Recognit. 2009 May-Jun;22 (3) :242-9. doi: 10.1002/jmr.937., URL: http://www.ncbi.nlm.nih.gov/pubmed/19177494, *Kienzle G, von Kempis J: CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12 (1) :73-82., URL: https://pubmed.ncbi.nlm.nih.gov/10607752/, *Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA: Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells. J Immunol. 2000 Sep 1;165 (5) :2903-10. , URL: https://pubmed.ncbi.nlm.nih.gov/10946324/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.

Species

Human, Non-human primates

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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