Anti-Hu CD138 Purified

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

A mixture of U266 and XG-1 human myeloma cell lines

Target Antigen

CD138

Clone

MI15

Applications

FC (QC tested), IP, WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Nadalin MR, Fregnani ER, Silva-Sousa YT, Perez DE: Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions. Braz Dent J. 2011;22 (3) :223-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/21915520, *Jourdan M, Caraux A, Caron G, Robert N, Fiol G, Rème T, Bolloré K, Vendrell JP, Le Gallou S, Mourcin F, De Vos J, Kassambara A, Duperray C, Hose D, Fest T, Tarte K, Klein B: Characterization of a transitional preplasmablast population in the process of human B cell to plasma cell differentiation. J Immunol. 2011 Oct 15;187 (8) :3931-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/21918187, *Noll JE, Vandyke K, Hewett DR, Mrozik KM, Bala RJ, Williams SA, Kok CH, Zannettino AC: PTTG1 expression is associated with hyperproliferative disease and poor prognosis in multiple myeloma. J Hematol Oncol. 2015 Oct 6;8:106., URL: https://pubmed.ncbi.nlm.nih.gov/26445238/, *Atanackovic D, Hildebrandt Y, Templin J, Cao Y, Keller C, Panse J, Meyer S, Reinhard H, Bartels K, Lajmi N, Sezer O, Zander AR, Marx AH, Uhlig R, Zustin J, Bokemeyer C, Kröger N: Role of interleukin 16 in multiple myeloma. J Natl Cancer Inst. 2012 Jul 3;104 (13) :1005-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/22745469, *Krishnan SR, Luk F, Brown RD, Suen H, Kwan Y, Bebawy M: Isolation of Human CD138 (+) Microparticles from the Plasma of Patients with Multiple Myeloma. Neoplasia. 2016 Jan;18 (1) :25-32., URL: https://pubmed.ncbi.nlm.nih.gov/26806349/, *Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A: Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999 Jan;104 (1) :152-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/10027728, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

Other References

*Hayashida K, Johnston DR, Goldberger O, Park PW: Syndecan-1 expression in epithelial cells is induced by transforming growth factor beta through a PKA-dependent pathway. J Biol Chem. 2006 Aug 25;281 (34) :24365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16807246?ordin, *Choi DS, Kim JH, Ryu HS, Kim HC, Han JH, Lee JS, Min CK: Syndecan-1, a key regulator of cell viability in endometrial cancer. Int J Cancer. 2007 Aug 15;121 (4) :741-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/17455248?ordin, *Manakil JF, Seymour GJ, Bartold PM: Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression. Oral Microbiol Immunol. 2007 Aug;22 (4) :272-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17600540?ordin, *Muto T, Miyoshi K, Munesue S, Nakada H, Okayama M, Matsuo T, Noma T: Differential expression of syndecan isoforms during mouse incisor amelogenesis. J Med Invest. 2007 Aug;54 (3-4) :331-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878683?ordin, *Ojeh N, Hiilesvuo K, Wärri A, Salmivirta M, Henttinen T, Määttä A: Ectopic expression of syndecan-1 in basal epidermis affects keratinocyte proliferation and wound re-epithelialization. J Invest Dermatol. 2008 Jan;128 (1) :26-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/17625591?ordina, *Jilani I, Wei C, Bekele BN, Zhang ZJ, Keating M, Wierda W, Ferrajoli A, Estrov Z, Kantarjian H, O'Brien SM, Giles FJ, Albitar M: Soluble syndecan-1 (sCD138) as a prognostic factor independent of mutation status in patients with chronic lymphocytic leukemia. Int J Lab Hematol. 2008 Jan 7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18190591?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody MI15 recognizes an extracellular epitope of CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.