Mouse IgG2b Isotype Control Purified

Product Specifications

Certification

RUO

Negative Species

Mouse,Rat,Pig,Human

Clone

PLRV219

Applications

FC (QC tested), IP, IHC-P, IHC-F, ICC, WB, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Harald Schwarz, Jan Gornicec, Theresa Neuper, Maria Alejandra Parigiani, Michael Wallner, Albert Duschl, and Jutta Horejs-Hoeck: Biological Activity of Masked Endotoxin. Sci Rep. 2017; 7: 44750. , URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC53577

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.

Applications Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.