Anti-Hu Myeloperoxidase Purified

Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human myeloperoxidase

Target Antigen

Myeloperoxidase

Clone

MPO421-8B2

Applications

FC (QC tested), ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Locke M, Francis RJ, Tsaousi E, Longstaff C: Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci Rep. 2020 Jul 16;10 (1) :11694., URL: https://pubmed.ncbi.nlm.nih.gov/32678135/

Other References

*Ximenes VF, Maghzal GJ, Turner R, Kato Y, Winterbourn CC, Kettle AJ: Serotonin as a physiological substrate for myeloperoxidase and its superoxide-dependent oxidation to cytotoxic tryptamine-4,5-dione. Biochem J. 2009 Dec 14;425 (1) :285-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/19828014, *Hansson M, Olsson I, Nauseef WM: Biosynthesis, processing, and sorting of human myeloperoxidase. Arch Biochem Biophys. 2006 Jan 15;445 (2) :214-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/16183032, *Ximenes VF, Silva SO, Rodrigues MR, Catalani LH, Maghzal GJ, Kettle AJ, Campa A: Superoxide-dependent oxidation of melatonin by myeloperoxidase. J Biol Chem. 2005 Nov 18;280 (46) :38160-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16148002, *Chooklin S, Pereyaslov A, Bihalskyy I: Pathogenic role of myeloperoxidase in acute pancreatitis. Hepatobiliary Pancreat Dis Int. 2009 Dec;8 (6) :627-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/20007081, *Murphy EJ, Maréchal A, Segal AW, Rich PR: CO binding and ligand discrimination in human myeloperoxidase. Biochemistry. 2010 Mar 16;49 (10) :2150-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/20146436

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.