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Anti-Hu CD111 Purified

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P) . Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Product Specifications

Certification

RUO

Immunogen

NIH/3T3 cells transfected with human CD111

Target Antigen

CD111

Clone

R1.302

Applications

FC (QC tested), IP, WB, IHC, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Cocchi F, Lopez M, Menotti L, Aoubala M, Dubreuil P, Campadelli-Fiume G: The V domain of herpesvirus Ig-like receptor (HIgR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D., URL: https://www.ncbi.nlm.nih.gov/pubmed/10627537, *Cocchi F, Lopez M, Dubreuil P, Campadelli Fiume G, Menotti L: Chimeric nectin1-poliovirus receptor molecules identify a nectin1 region functional in herpes simplex virus entry. J Virol. 2001 Sep;75 (17) :7987-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/11483743 , *Yoon M, Spear PG: Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 2002 Jul;76 (14) :7203-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12072519 , *Wang PY, Swain HM, Kunkler AL, Chen CY, Hutzen BJ, Arnold MA, Streby KA, Collins MH, Dipasquale B, Stanek JR, Conner J, van Kuppevelt TH, Glorioso JC, Grandi P, Cripe TP: Neuroblastomas vary widely in their sensitivities to herpes simplex virotherapy unrelated to virus receptors and susceptibility. Gene Ther. 2016 Feb;23 (2) :135-43., URL: https://pubmed.ncbi.nlm.nih.gov/26583803/, *Reymond N, Garrido-Urbani S, Borg JP, Dubreuil P, Lopez M: PICK-1: a scaffold protein that interacts with Nectins and JAMs at cell junctions. FEBS Lett. 2005 Apr 11;579 (10) :2243-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15811349

Other References

*Cocchi F, Lopez M, Dubreuil P, Campadelli Fiume G, Menotti L: Chimeric nectin1-poliovirus receptor molecules identify a nectin1 region functional in herpes simplex virus entry. J Virol. 2001 Sep;75 (17) :7987-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/11483743

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody R1.302 recognizes an extracellular epitope on CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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