Anti-Ms CD8b Purified

The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

Mouse CD8b

Target Antigen

CD8b

Clone

H35-17.2

Applications

IP, FC (QC tested), IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Cooney LA, Gupta M, Thomas S, Mikolajczak S, Choi KY, Gibson C, Jang IK, Danziger S, Aitchison J, Gardner MJ, Kappe SH, Wang R: Short-lived effector CD8 T cells induced by genetically attenuated malaria parasite vaccination express CD11c. Infect Immun. 2013 Nov;81 (11) :4171-81, URL: https://pubmed.ncbi.nlm.nih.gov/23980113/, *Weici Zhang, Masanobu Tsuda, Guo-Xiang Yang, Koichi Tsuneyama, Xiao-Song He, Aftab A. Ansari, William M. Ridgway, Ross L. Coppel, Zhe-Xiong Lian, Patrick S.C. Leung, M. Eric Gershwin: Lymphoma-Like T Cell Infiltration in Liver Is Associated with Increased Copy Number of Dominant Negative Form of TGFβ Receptor II. PLoS One. 2012; 7 (11) : e49413. , URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC34922, *Henderson AJ, Kumar A, Barnett B, Dow SW, Ryan EP: Consumption of rice bran increases mucosal immunoglobulin A concentrations and numbers of intestinal Lactobacillus spp. J Med Food. 2012 May;15 (5) :469-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/22248178, *Krieg C, Boyman O, Fu YX, Kaye J: B and T lymphocyte attenuator regulates CD8+ T cell-intrinsic homeostasis and memory cell generation. Nat Immunol. 2007 Feb;8 (2) :162-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17206146, *Chaudhary N, Datta K, Askin FB, Staab JF, Marr KA: Cystic fibrosis transmembrane conductance regulator regulates epithelial cell response to Aspergillus and resultant pulmonary inflammation. Am J Respir Crit Care Med. 2012 Feb 1;185 (3) :301-10., URL: https://pubmed.ncbi.nlm.nih.gov/22135344/, *Rubin B, Riond J, Leghait J, Gairin JE: Interactions between CD8alphabeta and the TCRalphabeta/CD3-receptor complex. Scand J Immunol. 2006 Sep;64 (3) :260-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/16918695

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody H35-17.2 recognizes an extracellular epitope of mouse CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on cytotoxic T cells and thymocytes.