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Anti-Hu CD101 Purified

CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human thymic clone B12

Target Antigen

CD101

Clone

BB27

Applications

FC (QC tested), IP, WB, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Bagot M, Martinel I, Charue D, Boulland ML, Wechsler J, Bensussan A, Boumsell L: Functional role of CD101 on skin dendritic cells. Adv Exp Med Biol. 1997;417:227-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/9286366 Bagot M, *Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debré P, Hosmalin A: Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 1998 Oct;64 (4) :484-93., URL: https://pubmed.ncbi.nlm.nih.gov/9766629/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Isotype

Mouse IgG1

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