Anti-Hu CD144 Purified

CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human endothelial cells

Target Antigen

CD144

Clone

55-7H1

Applications

FC (QC tested), IP, WB, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Tisato V, Zauli G, Voltan R, Gianesini S, di Iasio MG, Volpi I, Fiorentini G, Zamboni P, Secchiero P: Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One. 2012;7 (6) :e39543., URL: http://www.ncbi.nlm.nih.gov/pubmed/22737245 , *Agrawal V, Kelly J, Tottey S, Daly KA, Johnson SA, Siu BF, Reing J, Badylak SF: An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011 Dec;17 (23-24) :3033-44., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=agrawal+v+kelly+j+tottey+s , *De Ugarte DA, Alfonso Z, Zuk PA, Elbarbary A, Zhu M, Ashjian P, Benhaim P, Hedrick MH, Fraser JK: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow. Immunol Lett. 2003 Oct 31;89 (2-3) :267-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/14556988 , *Limaye V, Li X, Hahn C, Xia P, Berndt MC, Vadas MA, Gamble JR: Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members. Blood. 2005 Apr 15;105 (8) :3169-77, URL: http://www.ncbi.nlm.nih.gov/pubmed/15632208 , *Hamilton RD, Foss AJ, Leach L: Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007 Dec;211 (6) :707-16, URL: http://www.ncbi.nlm.nih.gov/pubmed/17922819

Other References

*Dejana E, Orsenigo F: Endothelial adherens junctions at a glance. J Cell Sci. 2013 Jun 15;126 (Pt 12) :2545-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/23781019

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.