Anti-Hu CD95 Purified

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

P815 cells transfected with human CD95

Target Antigen

CD95

Clone

EOS9.1

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Desbarats J, Birge RB, Mimouni-Rongy M, Weinstein DE, Palerme JS, Newell MK: Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nat Cell Biol. 2003 Feb;5 (2) :118-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/12545171 , *Matsuoka K, Kim HT, McDonough S, Bascug G, Warshauer B, Koreth J, Cutler C, Ho VT, Alyea EP, Antin JH, Soiffer RJ, Ritz J: Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation. J Clin Invest. 2010 May;120 (5) :1479-93, URL: http://www.ncbi.nlm.nih.gov/pubmed/20389017 , *Kasper HU, Konze E, Kern M, Stippel DL: CD95 and TNFα -induced apoptosis in liver metastases of colorectal carcinoma. In Vivo. 2010 Sep-Oct;24 (5) :653-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/20952729, *Conejo-Garcia JR, Benencia F, Courreges MC, Gimotty PA, Khang E, Buckanovich RJ, Frauwirth KA, Zhang L, Katsaros D, Thompson CB, Levine B, Coukos G: Ovarian carcinoma expresses the NKG2D ligand Letal and promotes the survival and expansion of CD28- antitumor T cells. Cancer Res. 2004 Mar 15;64 (6) :2175-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/15026360

Other References

*Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314 (3) :554-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/18078929?ordin, *Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106 (6) :2033-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/15941911?ordin, *Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170 (12) :6209-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/12794152?ordin, *Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17 (6) :1675-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/9501089?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody EOS9.1 recognizes an extracellular epitope of CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.