Anti-Hu CD160 Purified

CD160 is a cell surface glycoprotein of immunoglobulin superfamily, which functions as a costimulatory receptor expressed mainly on cytotoxic cell populations and recognizing both classical and non-classical MHC class I molecules. It can form disulfide-linked multimers. Down-modulation of CD160 occurs as a consequence of its proteolytic cleavage and the released soluble form was found to impair the MHC-class I specific cytotoxicity of CD8+ T lymphocytes and NK cells. In contrast to GPI-anchored isoform with broader expression among CD160 positive cells, expression of the transmembrane isoform is restricted to NK cells and is activation-dependent.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human NK cell line YT2C2

Target Antigen

CD160

Clone

BY55

Applications

FC (QC tested), IP

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Merino J, Ramírez N, Moreno C, Toledo E, Fernández M, Sánchez-Ibarrola A: BY55/CD160 cannot be considered a cytotoxic marker in cytomegalovirus-specific human CD8 (+) T cells. Clin Exp Immunol. 2007 Jul;149 (1) :87-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=merino+j+moreno+c+toledo+e , *Anumanthan A, Bensussan A, Boumsell L, Christ AD, Blumberg RS, Voss SD, Patel AT, Robertson MJ, Nadler LM, Freeman GJ: Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998 Sep 15;161 (6) :2780-90., URL: https://pubmed.ncbi.nlm.nih.gov/9743336/, *Maï za H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A: A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993 Sep 1;178 (3) :1121-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/7688788 , *Giustiniani J, Bensussan A, Marie-Cardine A: Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation. J Immunol. 2009 Jan 1;182 (1) :63-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/19109136 , *Bensussan A, Gluckman E, el Marsafy S, Schiavon V, Mansur IG, Dausset J, Boumsell L, Carosella E: BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity. Proc Natl Acad Sci U S A. 1994 Sep 13;91 (19) :9136-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/8090781

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody BY55 recognizes an extracellular epitope of CD160, a 27 kDa glycoprotein expressed on NK cells, NK-T cells, intestinal intraepithelial lymphocytes, TCR-gamma/delta T cells and a small population of TCR-alpha/beta T cells. The antibody detects both GPI-anchored and transmembrane form of CD160.