Anti-Ms LPAM-1 Purified

Integrin alpha 4 / beta 7 (CD49d / Ly69), also known as LPAM-1 (Llmphocyte Peyer´s patch adhesion molecule 1), is a heterodimeric (150 kDa / 130 kDa) integrin complex which mediates lymphocyte homing to Peyer´s patch high endothelial venules and to the intestinal lamina propria. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. Ligands of LPAM-1 are MAdCAM-1, VCAM-1, and fibronectin, but the alpha 4 subunit (CD49d) can mediate also homotypic adhesion.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

TK1 cells

Target Antigen

LPAM-1

Clone

DATK32

Applications

FC (QC tested), IP, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Priestley GV, Scott LM, Ulyanova T, Papayannopoulou T: Lack of alpha4 integrin expression in stem cells restricts competitive function and self-renewal activity. Blood. 2006 Apr 1;107 (7) :2959-67., URL: http://www.ncbi.nlm.nih.gov/pubmed/16357327 , *Riedel SS, Mottok A, Brede C, Bäuerlein CA, Jordán Garrote AL, Ritz M, Mattenheimer K, Rosenwald A, Einsele H, Bogen B, Beilhack A: Non-invasive imaging provides spatiotemporal information on disease progression and response to therapy in a murine model of multiple myeloma. PLoS One. 2012;7 (12) :e52398., URL: http://www.ncbi.nlm.nih.gov/pubmed/23300660

Other References

*Reese SR, Kudsk KA, Genton L, Ikeda S: l-selectin and alpha4beta7 integrin, but not ICAM-1, regulate lymphocyte distribution in gut-associated lymphoid tissue of mice. Surgery. 2005 Feb;137 (2) :209-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/15674203, , *Yang Y, Cardarelli PM, Lehnert K, Rowland S, Krissansen GW: LPAM-1 (integrin alpha 4 beta 7) -ligand binding: overlapping binding sites recognizing VCAM-1, MAdCAM-1 and CS-1 are blocked by fibrinogen, a fibronectin-like polymer and RGD-like cyclic peptides. Eur J Immunol. 1998 Mar;28 (3) :995-1004., URL: https://pubmed.ncbi.nlm.nih.gov/9541595/, *Altevogt P, Hubbe M, Ruppert M, Lohr J, von Hoegen P, Sammar M, Andrew DP, McEvoy L, Humphries MJ, Butcher EC: The alpha 4 integrin chain is a ligand for alpha 4 beta 7 and alpha 4 beta 1. J Exp Med. 1995 Aug 1;182 (2) :345-55., URL: http://www.ncbi.nlm.nih.gov/pubmed/7629498

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody DATK32 recognizes an extracellular epitope of integrin alpha 4 (CD49d) and integrin beta7 (Ly69) components of mouse LPAM-1 complex, which is expressed on the majority of peripheral lymphocytes, as well as on subsets of thymocytes and bone marrow cells.