Anti-Hu CD300e Purified

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

CD300e-HA-transfected cells

Target Antigen

CD300e

Clone

UP-H2

Applications

FC (QC tested), IP, WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173 (11) :6703-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=aguilar+h+orfao+a, *Brckalo T, Calzetti F, Pérez-Cabezas B, Borrà s FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40 (3) :722-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=brckalo+t+calzetti

Other References

*Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30 (5) :209-17., URL: http://www.ncbi.nlm.nih.gov/pubmed/19359216

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.