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Anti-Ms CD45R Purified

CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.

Product Specifications

Certification

RUO

Reactivity

Cat, Mouse, Human

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Target Antigen

CD45R

Clone

RA3-6B2

Applications

FC (QC tested), IP, IHC-P, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May;39 (5) :419-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/7514808, *Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May;48 (5) :653-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/10769049, *Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul;9 (1) :135-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/9697843, *Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr;69 (4) :249-59., URL: https://www.ncbi.nlm.nih.gov/pubmed/16538653, *Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar;7 (3) :293-301., URL: https://www.ncbi.nlm.nih.gov/pubmed/16429139, *Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1;172 (7) :4298-307., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=cappione+aj+anolik+jh , *Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. J Immunol. 2012 Sep 1;189 (5) :2488-501, URL: https://pubmed.ncbi.nlm.nih.gov/22851707/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 µg/ml

Isotype

Rat IgG2a kappa

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