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Anti-Ms CD105 Purified

CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Product Specifications

Certification

RUO

Immunogen

Inflamed mouse skin

Target Antigen

CD105

Clone

MJ7/18

Applications

FC (QC tested), IP, WB, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Ge AZ, Butcher EC: Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene. 1994 Jan 28;138 (1-2) :201-6., URL: https://pubmed.ncbi.nlm.nih.gov/8125301/, *Herouet C, Cottin M, LeClaire J, Enk A, Rousset F: Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells. In Vitr Mol Toxicol. 2000 Summer;13 (2) :113-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/11031322 , *Dassler K, Roohi F, Lohrke J, Ide A, Remmele S, Hütter J, Pietsch H, Pison U, Schütz G: Current limitations of molecular magnetic resonance imaging for tumors as evaluated with high-relaxivity CD105-specific iron oxide nanoparticles. Invest Radiol. 2012 Jul;47 (7) :383-91, URL: http://www.ncbi.nlm.nih.gov/pubmed/22659596 , *Rosu-Myles M, She YM, Fair J, Muradia G, Mehic J, Menendez P, Prasad SS, Cyr TD: Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLoS One. 2012;7 (6) :e38954. doi: 10.1371/journal.pone.0038954., URL: https://pubmed.ncbi.nlm.nih.gov/22719999/, *Cho SK, Bourdeau A, Letarte M, Zúñ iga-Pflücker JC: Expression and function of CD105 during the onset of hematopoiesis from Flk1 (+) precursors. Blood. 2001 Dec 15;98 (13) :3635-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=cho+sk+bourdeau+a , *Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25 (3B) :1851-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=warrington+k+hillarby+mc , *Fonsatti E, Nicolay HJ, Altomonte M, Covre A, Maio M: Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumours. Cardiovasc Res. 2010 Apr 1;86 (1) :12-9. doi: 10.1093/cvr/cvp332., URL: http://www.ncbi.nlm.nih.gov/pubmed/19812043

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody MJ7/18 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Species

Mouse

CAS Number

9007-83-4

Isotype

Rat IgG2a kappa

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