What is the catalog number for Anti-PCLO Purified?

The catalog number for Anti-PCLO Purified is 11-698-C100.

What sizes are available for Anti-PCLO Purified?

Anti-PCLO Purified is available in the following size(s): 0.1 mg.

Is Anti-PCLO Purified in stock?

Anti-PCLO Purified is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Anti-PCLO Purified?

Anti-PCLO Purified is manufactured by AFG-XBO.

What is the estimated delivery time for Anti-PCLO Purified?

Estimated delivery time for Anti-PCLO Purified is approximately 10 business day(s), depending on your location.

Anti-PCLO Purified

PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B) . In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human recombinant PCLO protein

Target Antigen

PCLO

Clone

PCLO-01

Applications

FC (QC tested), WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Dresbach T, Torres V, Wittenmayer N, Altrock WD, Zamorano P, Zuschratter W, Nawrotzki R, Ziv NE, Garner CC, Gundelfinger ED: Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment. J Biol Chem. 2006 Mar 3;281 (9) :6038-47., URL: http://www.ncbi.nlm.nih.gov/pubmed/16373352, , *Mukherjee K, Yang X, Gerber SH, Kwon HB, Ho A, Castillo PE, Liu X, Südhof TC: Piccolo and bassoon maintain synaptic vesicle clustering without directly participating in vesicle exocytosis. Proc Natl Acad Sci U S A. 2010 Apr 6;107 (14) :6504-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/20332206

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.

Applications Notes

Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.