Anti-gamma-Tubulin GCP2 Purified

Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs) . These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.

Product Specifications

Certification

RUO

Reactivity

Rat, Mouse, Pig, Human

Immunogen

GST-fusion protein containing amino acids 2-194 of mouse GCP2

Target Antigen

Gamma-Tubulin GCP2

Clone

GCP2-01

Applications

IP, WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Hořejší B, Vinopal S, Sládková V, Dráberová E, Sulimenko V, Sulimenko T, Vosecká V, Philimonenko A, Hozák P, Katsetos CD, Dráber P: Nuclear γ -tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol. 2012 Jan;227 (1) :367-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/21465471, *Dráberová E, D'Agostino L, Caracciolo V, Sládková V, Sulimenko T, Sulimenko V, Sobol M, Maounis NF, Tzelepis E, Mahera E, Křen L, Legido A, Giordano A, Mörk S, Hozák P, Dráber P, Katsetos CD: Overexpression and Nucleolar Localization of γ -Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol. 2015 Jul;74 (7) :723-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/26079448 , *Klebanovych A, Sládková V, Sulimenko T, Vosecká V, Čapek M, Dráberová E, Dráber P, Sulimenko V: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by protein tyrosine phosphatase SHP-1. Cells 2019 Apr 11;8 (4) :345., URL: https://pubmed.ncbi.nlm.nih.gov/30979083/, *Klebanovych A, Vinopal S, Dráberová E, Sládková V, Sulimenko T, Sulimenko V, Vosecká V, Macůrek L, Legido A, Dráber P: C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stress. Cells 2022 Feb 5;11 (3) :555., URL: https://pubmed.ncbi.nlm.nih.gov/35159364/, *Nejedlá M, Klebanovych A, Sulimenko V, Sulimenko T, Dráberová E, Dráber P, Karlsson R: The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 2020, Nov 12;4 (1) :e202000655., URL: https://pubmed.ncbi.nlm.nih.gov/33184056/, *Ivanova EL, Gilet JG, Sulimenko V, Duchon A, Rudolf G, Runge K, Collins SC, Asselin L, Broix L, Drouot N, Tilly P, Nusbaum P, Vincent A, Magnant W, Skory V, Birling MC, Pavlovic G, Godin JD, Yelcin B, Hérault Y, Dráber P, Chelly J, Hinckelmann MV: TUBG1 missense variants underlying cortical malformations disrupt neuronal locomotion and microtubule dynamics but not neurogenesis. Nat Commun. 2019 May 13;10 (1) :2129., URL: https://pubmed.ncbi.nlm.nih.gov/31086189/

Other References

*Bouissou A, Vérollet C, Sousa A, Sampaio P, Wright M, Sunkel CE, Merdes A, Raynaud-Messina B. {gamma}-Tubulin ring complexes regulate microtubule plus end dynamics. J Cell Biol. 2009 Nov 2;187 (3) :327-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/19948476

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.