Anti-BrdU Purified

*Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37 (10) :1517-27., URL: https://www.ncbi.nlm.nih.gov/pubmed/2476478, *O'Driscoll M, Jeggo PA: CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun;41 (11) :983-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18278071, *Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12;176 (6) :807-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/17353358, *Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67 (MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec;51 (12) :1681-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/14623936, *Lagares A, Li HY, Zhou XF, Avendaño C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J Neurosci. 2007 Jul 25;27 (30) :7939-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17652585, *Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep;289 (3) :C757-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/15857903, *Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr;29 (1) :33-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15954465, *Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb;294 (2) :H944-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/18083905, *Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37 (10) :1517-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/2476478

*Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107 (10) :819-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10504149?dopt=, *Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42 (7) :939-44. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8014477?dopt=A

The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.