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Anti-Hu Ig Lambda Light Chain Purified

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa) . Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Product Specifications

Certification

RUO

Immunogen

Human IgA1 lambda myeloma protein

Target Antigen

Ig Lambda Light Chain

Clone

1-155-2

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD: Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J Clin Immunol. 1982 Oct;2 (4) :264-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/6815220, *Bahler DW, Pindzola JA, Swerdlow SH: Splenic marginal zone lymphomas appear to originate from different B cell types. Am J Pathol. 2002 Jul;161 (1) :81-8., URL: https://pubmed.ncbi.nlm.nih.gov/12107092/, *Tierens AM, Holte H, Warsame A, Ikonomou IM, Wang J, Chan WC, Delabie J: Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type. Haematologica. 2010 Aug;95 (8) :1334-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/20145271

Other References

*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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