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Anti-Hu CD229 Purified

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Product Specifications

Certification

RUO

Immunogen

CD299-transfected 300.19 pre-B cell line

Target Antigen

CD229

Clone

HLy9.25

Applications

FC (QC tested), IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174 (11) :7033-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/15905546?itool, *de la Fuente MA, Tovar V, Villamor N, Zapater N, Pizcueta P, Campo E, Bosch J, Engel P: Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9. Blood. 2001 Jun 1;97 (11) :3513-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/11369645?itool, *Martín M, Del Valle JM, Saborit I, Engel P: Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule-associated protein binding receptor CD229. J Immunol. 2005 May 15;174 (10) :5977-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/15879090?itool

Other References

*Sintes J, Romero X, Marin P, Terhorst C, Engel P: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Exp Hematol. 2008 Sep;36 (9) :1199-204., URL: https://www.ncbi.nlm.nih.gov/pubmed/18495325?itool, *Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4) . Tissue Antigens. 2004 Aug;64 (2) :132-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/15245368?itool, *Bund D, Mayr C, Kofler DM, Hallek M, Wendtner CM: Human Ly9 (CD229) as novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (B-CLL) recognized by autologous CD8+ T cells. Exp Hematol. 2006 Jul;34 (7) :860-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16797413?itool, *Del Valle JM, Engel P, Martín M: The cell surface expression of SAP-binding receptor CD229 is regulated via its interaction with clathrin-associated adaptor complex 2 (AP-2) . J Biol Chem. 2003 May 9;278 (19) :17430-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/12621057?itool

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Applications Notes

Flow cytometry: Recommended dilution: 6 μg/ml; positive control: peripheral blood lymphocytes.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1

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