Anti-PRR7 Purified

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain) . Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Product Specifications

Certification

RUO

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Target Antigen

PRR7

Clone

TRAP3/10

Applications

WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286 (22) :19617-29, URL: http://www.ncbi.nlm.nih.gov/pubmed/21460222

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Applications Notes

Immunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer) .