Anti-Hu CD162 Purified

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human thymocytes

Target Antigen

CD162

Clone

TC2

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Wake H, Mori S, Liu K, Morioka Y, Teshigawara K, Sakaguchi M, Kuroda K, Gao Y, Takahashi H, Ohtsuka A, Yoshino T, Morimatsu H, Nishibori M: Histidine-Rich Glycoprotein Prevents Septic Lethality through Regulation of Immunothrombosis and Inflammation. EBioMedicine. 2016 Jul;9:180-194., URL: https://www.ncbi.nlm.nih.gov/pubmed/27333033, *Nakata K, Takeda S, Tanaka A, Kwang J, Komano J: Antiviral activity of acid beta-glucosidase 1 on enterovirus 71, a causative agent of hand, foot and mouth disease. J Gen Virol. 2017 Apr;98 (4) :643-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=nakata+k+takeda+s+tanaka+a+kwang+j

Other References

*Moore KL: Structure and function of P-selectin glycoprotein ligand-1. Leuk Lymphoma. 1998 Mar;29 (1-2) :1-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/9638971?ordina, *Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ. PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity. 1999 Sep;11 (3) :369-78., URL: https://www.ncbi.nlm.nih.gov/pubmed/10514015?ordin, *Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. J Immunol. 2000 Sep 1;165 (5) :2764-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/10946308?ordin, *Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. J Clin Immunol. 2004 Jan;24 (1) :62-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/14997035?ordin, *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002) ., URL: