Anti-Hu CD140b Purified

CD140b / PDGF-RB (platelet-derived growth factor receptor beta) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140b forms homodimers, or heterodimerizes with CD140a / PDGF-RA. Whereas CD140a can have both pro-proliferative or anti-proliferative effects, the CD140b induces in various cell types their proliferation and migration. CD140b has also developmental roles in the cardiovascular system and is preferentially expressed on some tumours such as medulloblastoma.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

CD140b-transfected NIH 3T3 cells

Target Antigen

CD140b

Clone

18A2

Applications

FC (QC tested), ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Fekete N, Rojewski MT, Fürst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H: GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One. 2012;7 (8) :e43255. doi: 10.1371/journal.pone.0043255, URL: https://www.ncbi.nlm.nih.gov/pubmed/22905242

Other References

*Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22 (10) :1276-312., URL: https://www.ncbi.nlm.nih.gov/pubmed/18483217?itool, *French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28 (18) :5646-57., URL: https://www.ncbi.nlm.nih.gov/pubmed/18606782?itool, *Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22 (23) :3255-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/19056881?itool, *Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11 (8) :732-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/19649203?itool

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The monoclonal antibody 18A2 recognizes an extracellular epitope of CD140b / PDGF-RB, the 180-190 kDa beta chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative roles.