Anti-Hu CD203c Purified

CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

HEK-293 cells transfected with human CD203c

Target Antigen

CD203c

Clone

NP4D6

Applications

FC (QC tested), ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol. 2001 Dec;126 (4) :335-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/11815741?itool, *Heneberg P, Riegerová K, Kučera P: Pimecrolimus is a potent inhibitor of allergic reactions to hymenopteran venom extracts and birch pollen allergen in vitro. PLoS One. 2015 Nov 12;10 (11) :e0142953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=heneberg+p+riegerov%C3%A1+k+ku%C4%8Dera+p , *Blatt K, Herrmann H, Mirkina I, Hadzijusufovic E, Peter B, Strommer S, Hoermann G, Mayerhofer M, Hoetzenecker K, Klepetko W, Ghanim V, Marth K, Füreder T, Wacheck V, Valenta R, Valent P: The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils. PLoS One. 2012;7 (1) :e29925. doi: 10.1371/journal.pone.0029925. Epub 2012 Jan 27., URL: https://www.ncbi.nlm.nih.gov/pubmed/22299028

Other References

*Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen-induced expression of CD203c on basophils predicts IgE-mediated wheat allergy. Allergol Int. 2009 Jun;58 (2) :193-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/19240377?itool, *Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy. J Allergy Clin Immunol. 2009 Apr;123 (4) :789-94.e20., URL: https://www.ncbi.nlm.nih.gov/pubmed/19348919?itool, *Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. Inflamm Res. 2009 Nov;58 (11) :755-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/19418203?itool, *Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3. 1. Blood. 2001 May 15;97 (10) :3303-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/11342463?itool, *Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999 Oct 1;94 (7) :2343-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/10498606?itool, *Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross-linking. Int J Immunopathol Pharmacol. 2008 Oct-Dec;21 (4) :797-806., URL: https://www.ncbi.nlm.nih.gov/pubmed/19144265?itool

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.