Anti-Ms CD79b Purified

CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1) . They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR) . CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Product Specifications

Certification

RUO

Negative Species

Human

Reactivity

Mouse

Immunogen

Purified CD79a/b (alpha/beta) dimers from WEHI231 cells

Target Antigen

CD79b

Clone

HM79

Applications

FC (QC tested), IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kouro T, Nagata K, Takaki S, Nisitani S, Hirano M, Wahl MI, Witte ON, Karasuyama H, Takatsu K: Bruton's tyrosine kinase is required for signaling the CD79b-mediated pro-B to pre-B cell transition. Int Immunol. 2001 Apr;13 (4) :485-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/11282988?itool, *Koyama M, Ishihara K, Karasuyama H, Cordell JL, Iwamoto A, Nakamura T: CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain. Int Immunol. 1997 Nov;9 (11) :1767-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9418137?ordina, *Maki K, Nagata K, Kitamura F, Takemori T, Karasuyama H: Immunoglobulin beta signaling regulates locus accessibility for ordered immunoglobulin gene rearrangements. J Exp Med. 2000 Apr 17;191 (8) :1333-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/10770800?ordin, *Li Y, Chen F, Putt M, Koo YK, Madaio M, Cambier JC, Cohen PL, Eisenberg RA: B cell depletion with anti-CD79 mAbs ameliorates autoimmune disease in MRL/lpr mice. J Immunol. 2008 Sep 1;181 (5) :2961-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/18713966?ordin, *Aiba Y, Kameyama M, Yamazaki T, Tedder TF, Kurosaki T: Regulation of B-cell development by BCAP and CD19 through their binding to phosphoinositide 3-kinase. Blood. 2008 Feb 1;111 (3) :1497-503., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025150?itool

Other References

*Kouro T, Nagata K, Takaki S, Nisitani S, Hirano M, Wahl MI, Witte ON, Karasuyama H, Takatsu K: Bruton's tyrosine kinase is required for signaling the CD79b-mediated pro-B to pre-B cell transition. Int Immunol. 2001 Apr;13 (4) :485-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/11282988?ordin, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/15661879?ordin, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/15240688?ordin, *Nagata K, Nakamura T, Kitamura F, Kuramochi S, Taki S, Campbell KS, Karasuyama H: The Ig alpha/Igbeta heterodimer on mu-negative proB cells is competent for transducing signals to induce early B cell differentiation. Immunity. 1997 Oct;7 (4) :559-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/9354476?ordina, *Teh YM, Neuberger MS: The immunoglobulin (Ig) alpha and Igbeta cytoplasmic domains are independently sufficient to signal B cell maturation and activation in transgenic mice. J Exp Med. 1997 May 19;185 (10) :1753-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9151700?ordina, *Gong S, Nussenzweig MC: Regulation of an early developmental checkpoint in the B cell pathway by Ig beta. Science. 1996 Apr 19;272 (5260) :411-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8602530?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex.