Anti-Hu CD326 Purified

*Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C: EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 2008 Mar;61 (3) :307-10., URL: http://www.ncbi.nlm.nih.gov/pubmed/17586680?ordina, *Winter MJ, Nagtegaal ID, van Krieken JH, Litvínov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163 (6) :2139-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/14633587?ordin, *Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J: Flow cytometric analysis of antigen expression in malignant and normal renal cells. Anticancer Res. 2000 Jul-Aug;20 (4) :2773-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/10953356?ordin, *Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A: Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors. Anticancer Res. 1998 Sep-Oct;18 (5B) :3669-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/9854475?ordina, *Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S: Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors. J Cutan Pathol. 1992 Feb;19 (1) :73-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1556271?ordina

The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Immunohistochemistry (paraffin sections) : Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. Immunocytochemistry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions. Flow cytometry: Recommended dilution: 1-3 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 mL lysate.