Anti-Ms CD8a Purified

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

Murine spleen cells

Target Antigen

CD8a

Clone

53-6.7

Applications

FC (QC tested), IP, IHC-P, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49 (5) :2172-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18263815?ordin, *Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103 (13) :5102-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/16549792?ordin, *Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177 (1) :306-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785526?ordin, *Kosy, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175 (5) :3309-17., URL: https://www.ncbi.nlm.nih.gov/pubmed/16116223?ordin, *Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109 (2) :183-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/11805130?ordin, *Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114 (4) :582-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15314695?ordin, *Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89 (12) :5557-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/1608966?ordina, *Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152 (2) :280-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6156984?ordina, *Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/398327?ordinal

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen) .

Applications Notes

Flow cytometry: Recommended dilution: 1.5 μg/ml. Immunohistochemistry (frozen sections) : Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.