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Anti-AGR2/AGR3 Purified

AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Purified human AGR3 protein

Target Antigen

AGR2/AGR3

Clone

AGR3.4

Applications

WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa) ; secreted cytoplasmic proteins which can serve as markers of carcinogenesis.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; Positive tissue: human colon. Western blotting: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line. Immunocytochemistry: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Isotype

Mouse IgG1

Frequently Asked Questions

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