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Anti-HLA-B7 Purified

HLA-B7 allele of human HLA class I major histocompatibility (MHC) antigen indicates higher risk of breast cancer and cervical cancer. Expression of HLA-B7 together with HLA-B27 is associated with increased susceptibility to spondyloarthropaties. Flow cytometry detection of these two alleles is being used to screen for patients, who suffer from inflammatory disorders affecting the sacroiliac and intervertebral joints, such as ankylosing spondylosis (AS) . The HLA-B7 antigen (11 alleles) is expressed in 22% of healthy Caucasian individuals.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

Papain solubilised HLA-A2, B7

Target Antigen

HLA-B7

Clone

BB7.1

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Nabel GJ, Nabel EG, Yang ZY, Fox BA, Plautz GE, Gao X, Huang L, Shu S, Gordon D, Chang AE: Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans. Proc Natl Acad Sci U S A. 1993 Dec 1;90 (23) :11307-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/8248244 , *Worley BS, van den Broeke LT, Goletz TJ, Pendleton CD, Daschbach EM, Thomas EK, Marincola FM, Helman LJ, Berzofsky JA: Antigenicity of fusion proteins from sarcoma-associated chromosomal translocations. Cancer Res. 2001 Sep 15;61 (18) :6868-75., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=worley+bs+goletz+tj , *Storkus WJ, Alexander J, Payne JA, Dawson JR, Cresswell P: Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc Natl Acad Sci U S A. 1989 Apr;86 (7) :2361-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/2784569 , *de la Salle H, Saulquin X, Mansour I, Klayme S, Fricker D, Zimmer J, Cazenave JP, Hanau D, Bonneville M, Houssaint E, Lefranc G, Naman R: Asymptomatic deficiency in the peptide transporter associated to antigen processing (TAP) . Clin Exp Immunol. 2002 Jun;128 (3) :525-31., URL: https://pubmed.ncbi.nlm.nih.gov/12067308/, *Lo Monaco E, Sibilio L, Melucci E, Tremante E, Suchà nek M, Horejsi V, Martayan A, Giacomini P: HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides. Immunol. 2008 Oct 15;181 (8) :5442-50., URL: http://www.ncbi.nlm.nih.gov/pubmed/18832701 , *Hofmann S, Glückmann M, Kausche S, Schmidt A, Corvey C, Lichtenfels R, Huber C, Albrecht C, Karas M, Herr W: Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by Nano-LC MALDI MS/MS. Mol Cell Proteomics. 2005 Dec;4 (12) :1888-97., URL: https://pubmed.ncbi.nlm.nih.gov/16112985/, *Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA-B7-restricted peptides of hTRT. Int Immunol. 2006 Dec;18 (12) :1707-18., URL: https://pubmed.ncbi.nlm.nih.gov/17077179/, *Rini BI, Selk LM, Vogelzang NJ: Phase I study of direct intralesional gene transfer of HLA-B7 into metastatic renal carcinoma lesions. Clin Cancer Res. 1999 Oct;5 (10) :2766-72., URL: https://pubmed.ncbi.nlm.nih.gov/10537340/

Other References

*Harjacek M, Margetić T, Kerhin-Brkljacić V, Martinez N, Grubić Z: HLA-B*27/HLA-B*07 in combination with D6S273-134 allele is associated with increased susceptibility to juvenile spondyloarthropathies. Clin Exp Rheumatol. 2008 May-Jun;26 (3) :498-504., URL: https://pubmed.ncbi.nlm.nih.gov/18578977/, *Wang SS, Wheeler CM, Hildesheim A, Schiffman M, Herrero R, Bratti MC, Sherman ME, Alfaro M, Hutchinson ML, Morales J, Lorincz A, Burk RD, Carrington M, Erlich HA, Apple RJ: Human leukocyte antigen class I and II alleles and risk of cervical neoplasia: results from a population-based study in Costa Rica. J Infect Dis. 2001 Nov 15;184 (10) :1310-4., URL: https://pubmed.ncbi.nlm.nih.gov/11679920/, *Grunewald J, Eklund A, Olerup O: Human leukocyte antigen class I alleles and the disease course in sarcoidosis patients. Am J Respir Crit Care Med. 2004 Mar 15;169 (6) :696-702., URL: https://www.ncbi.nlm.nih.gov/pubmed/14656748, *Bhattacharya P, Sengupta S: Predisposition to HPV16/18-related cervical cancer because of proline homozygosity at codon 72 of p53 among Indian women is influenced by HLA-B*07 and homozygosity of HLA-DQB1*03. Tissue Antigens. 2007 Oct;70 (4) :283-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/17767549

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody BB7.1 recognizes an extracellular antigen of HLA-B7 antigen. Although highly specific, it can cross-react with HLA-B42 antigen.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Isotype

Mouse IgG1

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