Anti-AHNAK1 Purified

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

Product Specifications

Certification

RUO

Reactivity

Human, Mouse

Immunogen

Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Target Antigen

AHNAK1

Clone

EM-09

Applications

IP (reported), WB (QC tested), IHC-F (verified), ICC (verified)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Johnson EK, Zhang L, Adams ME, Phillips A, Freitas MA, Froehner SC, Green-Church KB, Montanaro F: Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins. PLoS One. 2012;7 (8) :e43515. doi: 10.1371/journal.pone.0043515. (IP; Ms, Hu), URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=johnson+ek+zhang+l

Other References

*Sussman J, Stokoe D, Ossina N, Shtivelman E: Protein kinase B phosphorylates AHNAK and regulates its subcellular localization. J Cell Biol. 2001 Sep 3;154 (5) :1019-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/11535620?ordina, *Gentil BJ, Delphin C, Mbele GO, Deloulme JC, Ferro M, Garin J, Baudier J: The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B. J Biol Chem. 2001 Jun 29;276 (26) :23253-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11312263?ordina, *Haase H, Alvarez J, Petzhold D, Doller A, Behlke J, Erdmann J, Hetzer R, Regitz-Zagrosek V, Vassort G, Morano I: Ahnak is critical for cardiac Ca (V) 1.2 calcium channel function and its beta-adrenergic regulation. FASEB J. 2005 Dec;19 (14) :1969-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/16319140?ordina, *Matza D, Badou A, Kobayashi KS, Goldsmith-Pestana K, Masuda Y, Komuro A, McMahon-Pratt D, Marchesi VT, Flavell RA: A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation. Immunity. 2008 Jan;28 (1) :64-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/18191595?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Applications Notes

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required. Western blotting: Recommended dilution: 1 μg/ml, non-reducing conditions