Anti-CRP Purified

The C-reactive protein (CRP) is a cyclic pentameric pentraxin family acute phase protein composed of five identical noncovalently bound nonglycosylated subunits (each subunit 24 kDa; physiologic CRP molecule 117,5 kDa) . CRP is produced by the liver and its plasma levels rise dramatically during inflammatory processes occuring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.) and is also believed to play an important role in innate immunity. Patients with elevated basal levels of CRP are at increased risk for hypertension and cardiovascular disease.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Purified human CRP

Target Antigen

CRP

Clone

Applications

ELISA, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Meyer MH, Hartmann M, Krause HJ, Blankenstein G, Mueller-Chorus B, Oster J, Miethe P, Keusgen M: CRP determination based on a novel magnetic biosensor. Biosens Bioelectron. 2007 Jan 15;22 (6) :973-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hartmann+m+blankenstein, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

Other References

*van der Harst P, Voors AA, Volbeda M, Buikema H, van Veldhuisen DJ, van Gilst WH: Usefulness of preoperative C-reactive protein and soluble intercellular adhesion molecule-1 level for predicting future cardiovascular events after coronary artery bypass grafting. Am J Cardiol. 2006 Jun 15;97 (12) :1697-701., URL: https://pubmed.ncbi.nlm.nih.gov/16765116/, *Meyer MH, Hartmann M, Krause HJ, Blankenstein G, Mueller-Chorus B, Oster J, Miethe P, Keusgen M: CRP determination based on a novel magnetic biosensor. Biosens Bioelectron. 2007 Jan 15;22 (6) :973-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hartmann+m+blankenstein

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody C2 recognizes human CRP, an 117 kDa plasma acute phase protein; it recognizes the antigen both in the presence and in the absence (samples containing EDTA) of Ca2+.