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Anti-Cyclin D1 Purified

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

Product Specifications

Certification

RUO

Immunogen

Purified cyclin D1 protein

Target Antigen

Cyclin D1

Clone

CD1.1

Applications

IP, WB, IHC-P, IHC-F, ICC, ELISA, FC-IC

Concentration

1 mg/mL

Format

Purified

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Pucelik B, Barzowska A, Czarna A: DYRK1A inhibitors leucettines and TGF-β inhibitor additively stimulate insulin production in beta cells, organoids, and isolated mouse islets. PLoS One. 2023 May 17;18 (5) :e0285208., URL: https://pubmed.ncbi.nlm.nih.gov/37195917/

Other References

*Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11 (8) :957-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9136925?ordina, *Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12 (22) :3499-511., URL: https://www.ncbi.nlm.nih.gov/pubmed/9832503?ordina, *Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282 (6) :3799-808., URL: https://www.ncbi.nlm.nih.gov/pubmed/17130121?ordin, *Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6, URL: https://www.ncbi.nlm.nih.gov/pubmed/18328826?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Species

Human, Rat

CAS Number

9007-83-4

Isotype

Mouse IgG1

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