Anti-Hu CD79a Purified

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta) . They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR) . CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Product Specifications

Certification

RUO

Reactivity

Chicken, Rabbit, Opossum, Guinea pig, Horse, Cow, Pig, Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Target Antigen

CD79a

Clone

HM57

Applications

FC (QC tested), IHC-F (reported), IHC-P (reported)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86 (4) :1453-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/7632952?ordinal, *Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY: Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150 (12) :5429-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/8515069?ordinal, *van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J: The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146 (11) :3881-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/2033258?ordinal, *Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ: The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation. Eur J Immunol. 1992 Oct;22 (10) :2753-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/1396979?ordinal, *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119 (1-2) :56-62. , URL: http://www.ncbi.nlm.nih.gov/pubmed/17673300 , *Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147 (11) :2474-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/1747162?ordinal, *Faldyna M, Sinkora J, Knotigova P, Rehakova Z, Moravkova A, Toman M: Flow cytometric analysis of bone marrow leukocytes in neonatal dogs. Vet Immunol Immunopathol. 2003 Oct 15;95 (3-4) :165-76. doi: 10.1016/s0165-2427 (03) 00135-1. (dog), URL: https://pubmed.ncbi.nlm.nih.gov/12963277/

Other References

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a) - and Igbeta (CD79b) -mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177 (11) :7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina, *Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172 (4) :2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina, *Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194 (11) :1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Applications Notes

Flow cytometry: Recommended dilution: 5 μg/ml. Intracellular staining.Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml.Immunohistochemistry (frozen sections) : Recommended dilution: 10 μg/ml.