What is the catalog number for Anti-LST1 Purified?

The catalog number for Anti-LST1 Purified is 11-528-C100.

What sizes are available for Anti-LST1 Purified?

Anti-LST1 Purified is available in the following size(s): 0.1 mg.

Is Anti-LST1 Purified in stock?

Anti-LST1 Purified is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Anti-LST1 Purified?

Anti-LST1 Purified is manufactured by AFG-XBO.

What is the estimated delivery time for Anti-LST1 Purified?

Estimated delivery time for Anti-LST1 Purified is approximately 10 business day(s), depending on your location.

Anti-LST1 Purified

LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes) . At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1

Target Antigen

LST1

Clone

LST1/02

Applications

IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Draber P, Stepanek O, Hrdinka M, Drobek A, Chmatal L, Mala L, Ormsby T, Angelisova P, Horejsi V, Brdicka T: LST1/A is a myeloid leukocyte-specific transmembrane adaptor protein recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to the plasma membrane. J Biol Chem. 2012 Jun 29;287 (27) :22812-21, URL: https://www.ncbi.nlm.nih.gov/pubmed/22589543

Other References

*Rollinger-Holzinger I, Eibl B, Pauly M, Griesser U, Hentges F, Auer B, Pall G, Schratzberger P, Niederwieser D, Weiss EH, Zwierzina H: LST1: a gene with extensive alternative splicing and immunomodulatory function. J Immunol. 2000 Mar 15;164 (6) :3169-76., URL: https://pubmed.ncbi.nlm.nih.gov/10706707/, *Yu X, Weissman SM: Characterization of the promoter of human leukocyte-specific transcript 1. A small gene with a complex pattern of alternative transcripts. J Biol Chem. 2000 Nov 3;275 (44) :34597-608., URL: http://www.ncbi.nlm.nih.gov/pubmed/10944527?ordina, *Raghunathan A, Sivakamasundari R, Wolenski J, Poddar R, Weissman SM: Functional analysis of B144/LST1: a gene in the tumor necrosis factor cluster that induces formation of long filopodia in eukaryotic cells. Exp Cell Res. 2001 Aug 15;268 (2) :230-44., URL: http://www.ncbi.nlm.nih.gov/pubmed/11478849?ordina, *Mulcahy H, O'Rourke KP, Adams C, Molloy MG, O'Gara F: LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection. Immunogenetics. 2006 Jan;57 (12) :893-903., URL: http://www.ncbi.nlm.nih.gov/pubmed/16362817?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Applications Notes

Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line. Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.