Anti-SHIP1 Purified

SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI (3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Peptide coresponding to a sequence within N-terminal domain of human SHIP-1.

Target Antigen

SHIP1

Clone

SHIP-01

Applications

FC-IC (QC tested), WB (verified), ICC (reported)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Lo TC, Barnhill LM, Kim Y, Nakae EA, Yu AL, Diccianni MB: Inactivation of SHIP1 in T-cell acute lymphoblastic leukemia due to mutation and extensive alternative splicing. Leuk Res. 2009 Nov;33 (11) :1562-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/19473701

Other References

*Harris SJ, Parry RV, Westwick J, Ward SG: Phosphoinositide lipid phosphatases: natural regulators of phosphoinositide 3-kinase signaling in T lymphocytes. J Biol Chem. 2008 Feb 1;283 (5) :2465-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18073217?ordin, *Xing W, Hamaguchi M: Effects of SHIP-1 on MMP2 secretion and invasion of SR3Y1 cells. J Genet Genomics. 2007 Apr;34 (4) :285-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/17498626?ordin, *Tarasenko T, Kole HK, Chi AW, Mentink-Kane MM, Wynn TA, Bolland S: T cell-specific deletion of the inositol phosphatase SHIP reveals its role in regulating Th1/Th2 and cytotoxic responses. Proc Natl Acad Sci U S A. 2007 Jul 3;104 (27) :11382-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/17585010?ordin, *Ai J, Maturu A, Johnson W, Wang Y, Marsh CB, Tridandapani S: The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1. Blood. 2006 Jan 15;107 (2) :813-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/16179375?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen) . Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.

Applications Notes

Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.