Anti-PKAc Purified

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; proteinkinase A catalytic subunit) and two regulatory subunits (PKAr) . Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit – PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha) .

Target Antigen

PKAc

Clone

6D2.1

Applications

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 24;97 (22) :11893-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/11027313?ordin, *Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 24;99 (26) :16800-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/12482947?ordin, *Komagiri Y, Kitamura N: Comparison of effects of PKA catalytic subunit on I (h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug;28 (4) :177-89., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878598?ordin, *Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ: Transgenic inhibitors identify two roles for protein kinase A in Drosophila development. Genetics. 1999 May;152 (1) :281-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10224260?ordin, *Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol Endocrinol. 1990 Mar;4 (3) :465-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/2342480?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa) . The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Applications Notes

Western blotting: Recommended dilution: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis) .