Anti-CTBP1 Purified

C-terminal binding protein 1 / brefeldin A (BFA) -dependent ADP-ribosylation substrate (CtBP1/BARS) is a highly conserved transcriptional corepressor, that plays a critical role in regulating gene expression patterns throughout development and in oncogenesis. CtBP regulates target gene transcription, in part, via recruitment of histone deacetylases involved in gene silencing and may act to coordinate histone modifications that regulate gene expression.

Product Specifications

Certification

RUO

Reactivity

Mouse, Human

Immunogen

Bacterially expressed full-length human CtBP1

Target Antigen

CTBP1

Clone

PAb (511)

Applications

IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25 (11) :2326-37., URL: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=16724116&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

Other References

*Valenta T, Lukáš J, Kořínek V: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31 (9) :2369-80., URL: https://pubmed.ncbi.nlm.nih.gov/12711682/, *Nadauld LD, Phelps R, Moore BC, Eisinger A, Sandoval IT, Chidester S, Peterson PW, Manos EJ, Sklow B, Burt RW, Jones DA: Adenomatous polyposis coli control of C-terminal binding protein-1 stability regulates expression of intestinal retinol dehydrogenases. J Biol Chem. 2006 Dec 8;281 (49) :37828-35., URL: https://pubmed.ncbi.nlm.nih.gov/17028196/, *Lopez-Garcia J, Periyasamy M, Thomas RS, Christian M, Leao M, Jat P, Kindle KB, Heery DM, Parker MG, Buluwela L, Kamalati T, Ali S: ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. 2006; 34 (21) :6126-36., URL: https://pubmed.ncbi.nlm.nih.gov/17085477/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The polyclonal antibody reacts with CtBP1, a 49 kDa transcriptional corepressor (intracellular antigen), that plays a critical role in regulating gene expression.